Hello,
is it necassary to put the already closed vial in an autoclave after filtered with a 0.22 filter (bottle top)? Just wondering because I read about it... |
| Reply » double sterilisation? |
no.
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| Reply » double sterilisation? |
Not necessary. I only use a 0.45 micron filter and have had no problems.
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| Reply » double sterilisation? |
Why the 0.45? Ive heard there is some bacteria which cant be filtered with this size...
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| Reply » double sterilisation? |
Quote: | Why the 0.45? Ive heard there is some bacteria which cant be filtered with this size... | if it aint broke, dont try to fix it. .45's have been the main filter size used by the majority for as long as with no problems
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| Reply » double sterilisation? |
Quote: | Why the 0.45? Ive heard there is some bacteria which cant be filtered with this size... | Guys use .45 and don't have any problems in the real world. But if you want to use .20/.22, then go ahead. I do.
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